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KMID : 0364820120480030225
Korean Journal of Microbiology
2012 Volume.48 No. 3 p.225 ~ p.227
Decolorization of a Dye by Immobilized Lignin Degrading Enzymes Generated from Transformants of Merulius tremellosus Fr
Min Dong-Suk

You Seon-Hwa
Kim Myung-Kil
Choi Hyoung-T.
Abstract
Lignin degrading enzymes from white rot fungi show broad substrate specificities, and therefore they can degrade
variety of recalcitrant compounds. We have used three different protocols for the generation of immobilized laccase
and manganese peroxidase crude enzymes from the genetically transformed strains of Merulius tremellosus Fr.
These immobilized enzymes were used in the decolorization of Remazol Brilliant Blue R (RBBR), and they showed
about 75% decolorization rates during the 48 h reactions. Although the decolorization efficiency decreased by
10?15% after a repeated use of the immobilized enzymes, these can be reused in various degrading reactions
KEYWORD
Merulius tremellosus, decolorization, immobilized enzyme, laccase, manganese peroxidase
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